Recent Science Lab Questions

3
Votes

Plating cells at the right concentration

I have 1.6x10^6 uL cells/mL in 60 mL media and want to plat them at 500,00 uL/mL per plate how wo...

cells plate
1
Votes

Is there a way to compute restriction mapping on a computer?

It's really tedious to do restriction mapping by hand!

restriction mapping
1
Votes

Flow cytometry buoyant cells

After I centrifuge my cell in the FACTS buffer the cell pellet seems extremely buoyant for some r...

flow cytometry centrifuge
2
Votes

Bacteria?

Hello, I am working with a breast cancer cell line and noticed today some black dots on my plat...

bacteria breast cancer
1
Votes

DNA spectrophotometry

How do I find the amount of ug of DNA I have from a spectrophotometer reading?

dna spectrophotometer
0
Votes

How do I dilute my qRTPCR primers?

I am unsure about how to dilute my qRTPCR Primers. Can someone help me!!!

primers
3
Votes

What does a RNA Prep Values 260/280 value mean?

My RNA prep had a 260/280 value higher than 2, what does this mean?

rna prep
0
Votes

How to start a qRT PCR primer design?

Hi, I am trying to create a primer for a qRTPCR reaction but do not know where to start.

pcr primer
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