Plating cells at the right concentration
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I have 1.6x10^6 uL cells/mL in 60 mL media and want to plat them at 500,00 uL/mL per plate how would I go about doing this?
Answers
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M1V1=M2V2 I love this equation :)!!!
M1- cells/mL you have (1.6 x10^6)
V1- how much should go in the desired amount of media (unknown)
M2- cells/mL you need (500,000)
V1- desired amount of total media (number of plates x ML of total media per plate)
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