Flow cytometry buoyant cells

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After I centrifuge my cell in the FACTS buffer the cell pellet seems extremely buoyant for some reason. I am wondering if there is anyway to prevent this or what the best way to remove the supernatant would be.

flow cytometry centrifuge
Asked by B14lab

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James writes:

I would use a pipet to remove it, and pipet very slowly!

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joey78 writes:

I think if the cells are undergoing apoptosis they may becoming more buoyant :o

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Wayne B writes:

After the fixation step the cells tend to become more buoyant. You may want to consider spinning the cells down again at a slightly higher or longer RMP.

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